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Image Search Results
Journal: bioRxiv
Article Title: Cryo-EM Structure of AAA + ATPase Thorase Reveals Novel Helical Filament Formation
doi: 10.1101/2024.11.22.624887
Figure Lengend Snippet: (A) Domain diagram of Mouse Thorase. The construct used in this study comprised residues 41-361. (B) Size exclusion chromatography elution profile of Thorase Monomer. (C and D) Preparation of Thorase filaments by incubation with non-hydrolysable ATP Analog (ATP-γ-S) (E) Negative stain electron micrograph of the Thorase in running buffer with the addition of 1.0 mM ATP-γ-S in which filaments (black arrows) are readily distinguished (F) Cryo-EM micrograph of the Thorase in running buffer with the addition of 1.0 mM ATP-γ-S in which filaments in vitreous ice (black arrows) are readily distinguished.
Article Snippet:
Techniques: Construct, Size-exclusion Chromatography, Incubation, Staining, Cryo-EM Sample Prep